One of the major concerns regarding automated nucleic acid extraction is the risk of cross-contamination, which occurs when negative specimens are contaminated by positive ones. Possible causes of cross-contamination include aerosols, leaks, and faulty robotics. While this problem is unlikely to arise when using the MagNA Pure, BioRobot M48, or other similarly priced automated extraction systems, it should be noted that cross-contamination is not always prevented. To minimize the risk of cross-contamination, users can upgrade the software of the machines and change tips when necessary. Furthermore, automated extraction improves consistency and reliability.
The highly sensitive nature of nucleic acids makes it crucial to extract samples properly, allowing for greater consistency and reliability. Because nucleic acids undergo sensitive quantitative techniques like qPCR, minor variations in sample preparation can affect downstream analysis. This means that automated nucleic acid extraction can improve research results by improving the quality and consistency of samples. To achieve these goals, researchers should evaluate the benefits of automated extraction. The mTitan system was operated using a laptop computer with software similar to that used for CNC milling. This software uses G-code to program the device and provides instructions on movement, temperature, and amount of material to deposit. It can process up to 6 samples at once. Click here for more information.
In addition to this, the mTitan uses a magnetic coupling, similar to that of a fish tank cleaner. This feature allows the system to isolate the nucleic acid without any direct contact. Automated extraction via the m1000 system can offer high reliability, and it reduces the hands-on time of the researcher. However, this automated system isn't cheap. In fact, it costs more than a manual extraction method. The m1000 reagents are expensive. But, the cost-to-quality ratio for an m1000 automated extraction system is higher than that of a manual one. In addition, the sample that was detected with the automated method was also positive, indicating the presence of the virus.
The VERSA NAP automated DNA/RNA extraction system is compatible with multiple commercial kits, reagents, and other labware. These systems also allow users to update their workflows as new consumables become available. One example of a successful use of an automated DNA/RNA extraction system is a case study of COVID-19 sample preparation in a hospital in Minsk, Belarus. This user's validation poster demonstrated reproducibility of the automated viral RNA extraction process on COVID-19 soil samples. Automated nucleic acid extraction is a viable alternative to manual methods that are labor-intensive and slow.
The BioRobot M48 instrument from Qiagen, the MagNA Pure method from Roche Applied Sciences, and the QIAamp Viral RNA Mini kit from Invitrogen are examples of such systems. The latter two instruments have been tested in vitro for sensitivity of the PCR using poliovirus type 2 Sabin cerebrospinal fluid. The BioRobot M48 instrument was capable of detecting all 18 replicates of 100 PFU/ml by both methods. The mTitan system is a programmable, robust nucleic acid extraction instrument. It can perform NA amplification and heated incubation. The mTitan system has been tested in the laboratory setting and has exhibited equal performance to a similar commercially available automated nucleic acid extraction system. Hence one should look for the best laboratory equipment company.
In addition, the mTitan system was validated in the Yellowstone National Park. All of these tests demonstrate the mTitan system's potential as a high-performance, low-cost automated nucleic acid extraction solution. For more info, check out this related link: https://en.wikipedia.org/wiki/Medical_laboratory.
